Bovine serum albumin is noncovalently linked to the external surface of mesoporous silica nanoparticles through the avidin-biotin bridge with a cleavable ligand . This material is called (BSA-B)-Avidin-Biotin-SS-AP-FITC-MSN. For further study, it is labeled with FITC dye and has a cleavable disulfide bond. This system was designed for how the protein functionalization on MSN surface influences the endocytosis efficiency of mesoporous silica nanoparticles toward cancer cell HeLa. We focus on how the dual layer protein deposition on MSN surface change endocytosis behaviour toward cells. Those materials were treated with DTT and enzyme trypsin in order to study the difference between protein on surface and protein in the solution. Avidin-Biotin-SS-AP-FITC-MSN has greater affinity for biotinylated BSA than native MSN. Once BSA is functionalized on MSN surface, it shows the opposite behavior for Avidin-Biotin-SS-AP-FITC-MSN. Thus, we can manipulate the endocytotic behavior of MSN by simply linking protein or other biomolecule on MSN.

Membrane trafficking effect of protein functionalized mesoporous silica nanoparticles

243rd National Meeting (2012)

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