Analytical methods for the detection of undeclared food allergens serve many purposes.  In food manufacturing the methods are used to confirm identity, quality of ingredients, and effectiveness of sanitation processes. For regulatory agencies, the methods are often needed to determine the presence and identity of an undeclared allergen. Unlike the former, the latter often requires screening for more than one allergen. Multiplex assays provide a cost- and time-effective approach whereby the identification, quantitation, and confirmation of the presence of an allergenic food can be combined into a single analysis. Unlike allergen-specific assays, which focus on optimizing sensitivity, multiplex assays employ procedures designed to reliably work with several analytes and rely on numerous end points to increase confidence in the results generated. The application of multiplex methods to foods will be discussed.

Multiplex immunochemical approaches for detection of food allergens

243rd National Meeting (2012)

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