Anthropogenic estrogens in natural waters behave as endocrine disruptors in aquatic species and in humans.  Current analyses involve solutions that inherently contain humic substances, but such substances bind with estrogens and interfere with estrogen concentration estimates, especially in compounds present in water effluents.  To accurately survey estrogen concentrations, the long-term interaction between estrogens and humic substances must be determined. This study characterizes binding between estrogens and humic substances using Saturation Transfer Double Difference (STDD) NMR to isolate the spectrum of the bound species. We will present NMR spectra of low-concentration estrogens and humic substances in aqueous solution using a solvent suppression pulse program, as well as some initial STDD studies focusing on binding interaction in deuterated water.  In the long run, this study aspires to answer questions surrounding the validity of current estrogen analytical techniques at various concentration levels and persistence of these levels post consumption.

Analysis of binding between estrogenic compounds and humic substances using STDD-NMR with water suppression

243rd National Meeting (2012)

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