The potential benefits chromatographic media featuring two distinct functional regions spatially separated from one another within the same support bead have been clearly demonstrated in the context of 'nanoplex' purification, and fluidised bed separation of organic acids and proteins. However, all bi-layered supports made thus far, fall short of the ideal design brief of a thin inert outer size excluding layer, freely accessible to smaller components, but not larger entities, surmounting a fully functionalised adsorptive core. In this presentation, we describe our recent progress on the development of improved bi-layered, bi-functional supports. Specifically we report on the use of: 'bottom-up' microwave-assisted and viscosity enhanced 'reaction-diffusion balancing' techniques, which permit different functionalities to be built into underivatized SEC matrices as discrete 'onion-like' layers; and 'top-down' plasma methods, which allow one to selectively modify the exteriors of commercial adsorbents, either by shaving ligands away and/or burying them beneath a thin polymer graft.

Improved bi-layered bi-functional chromatography matrices

243rd National Meeting (2012)

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