Layered beads is a completely new design concept for bioprocess chromatography media. The technology is based on beads functionalized in layers that gives the beads interesting chromatographic properties and new features compared to traditional chromatography media. One example of a layered bead is called Core Beads, where ligands are situated exclusively in the interior of the bead without any functionality in the outer layer. This design enables the bead to have a molecular weight cutoff determined by the bead pore size and a functionality determined by the ligand in the core. This type of media is interesting for flowthrough purification of large molecules such as viruses and plasmid DNA.Another type of layered bead is called Shell Beads. In this case the ligands are situated exclusively in the outer shell of the particles. This leads to highly improved mass transfer and enables higher resolution than traditional chromatography media with the same particle size. Shell Beads are an interesting alternative for any polishing step.The design concept of layered beads will be presented along with application examples that illustrate the potential uses of layered beads.

Layered beads: A new purification tool in downstream processing of biomolecules

243rd National Meeting (2012)

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