In anion exchange flow-through (FT-AIEX) applications membrane chromatography offers several advantages over traditional bead columns. Conventional quaternary amine based chemistries require typically low feed conductivity and involves often dilution of feed streams, which can result in facility fit limitations when high titer processes are accommodated in existing plants. To address these limitations and facilitate a wider design space for FT-AIEX membrane chromatography at commercial scale, a new generation of positively charged salt tolerant membrane adsorbers (Sartobind STIC�) was developed. Additionally, an ultrascale down device was developed to minimize the feedstock requirements for process development, characterization and validation.  In this study, the performance of Sartobind STIC� was explored for host cell protein clearance using industrially relevant MAb feedstreams at different experimental conditions. The membrane adsorber was also tested for clearance of model viruses spiked in MAb feedstreams.  Data demonstrating the scalability of ultrascale down device to process scale will also be presented.

Next generation anion exchange membrane adsorbers for downstream processing

243rd National Meeting (2012)

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