Lysozyme was attached through an amide linkage between peptide aspartate and glutamate residues to amino-glycine-cellulose derived from preparations of cotton nanocrystals. A carbodiimide-activation coupling reaction was used to create the lysozyme-cellulose conjugates in a polar solvent giving yields of lysozyme at 400 mg/gram cotton. Nano-crystalline preparations were produced through acid hydrolysis and mechanical breakage of the cotton fibers from a scoured and bleached cotton fabric and a scoured and bleached, mercerized fabric, which was shown to produce cellulose I (NCI) and cellulose II (NCII) crystals respectively. Scanning electron microscopic images of the enzyme conjugates of NCII also revealed uniformly smaller particles than the NCI counterparts. The incorporation of lysozyme conjugated to the NCI and NCII preparations was up to 10 fold greater than lysozyme covalently conjugated to whole cotton fabric while also retaining very high lysozyme activity (1500 U/mg cotton).

Immobilization of lysozyme-cellulose amide-linked conjugates on cellulose I and II cotton nanocrystalline preparations

243rd National Meeting (2012)

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