Several microRNAs have been linked to the occurrence of cancer, and thus the accurate measurement of these short, regulatory sequences could potentially serve as an early marker of disease.  We aim to develop a portable electrochemical sensor for the detection of relative miRNA levels in normal and diseased cells. Using a combination of surface chemistry and microfluidic technology, our design construct allows for both high-throughput screening and quantitative assessment of specific sequences. Hybridization events at thiolated gold surfaces are measured indirectly by the downstream electrochemical detection of displaced redox-active tags. Preliminary results of surface-bound competition assays with ferrocene-modified oligonucleotides and sample target sequences will be presented.

Development of an electrochemical sensor for microRNA levels

243rd National Meeting (2012)

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