pH sensitive hydrogel microparticles have shown potential as an antigen carrier and further investigation on the effect of targeting and activating antibodies on the surface of particles was needed. Herein, we report microparticles encapsulating ovalbumin at 40% efficiency and decorated with DEC205, CD40 and/or HA antibodies. The activity of the HA antibody was confirmed by measuring its interaction with the corresponding peptide. Cell uptake studies showed uptake and presentation of ovalbumin by BMDC in-vitro as well as dendritic cells and monocytes in-vivo irrespective of the type of antibody on the particle surface. Further investigation of CD80 and CD86 upregulation didn't show the effect of antibodies in both in-vitro and in-vivo. Further in-vivo ovalbumin-expressing vesicular stomatitis virus (VSV-OVA) challenge studies showed expansion of cytokine-producing CD8 T cells using two different strategies, accelerated vaccination using pre-loaded BMDC and a traditional mouse immunization setting.

Targeting and activating antibodies were not essential for activation of dendritic cells by ovalbumin encapsulated in pH sensitive hydrogel microparticles

243rd National Meeting (2012)

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