Trisulfide has been detected as a source of molecular heterogeneity in protein therapeutics. Chromatography studies using several different proteins demonstrated that a cysteine (Cys)-containing wash, performed after loading of the protein and prior to elution, converted the protein trisulfide bonds to native disulfides. The Cys wash is a convenient method for trisulfide conversion, and the studies illustrated the method to be robust under a range of processing conditions relevant for the manufacturing of protein therapeutics.

Robustness of on-column trisulfide elimination from protein therapeutics

243rd National Meeting (2012)

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