The growing demand for clinical-scale numbers of human mesenchymal stem cells (MSC) for cellular therapies requires a large-scale, fully monitored and controlled culture system for MSC production. Here we report the expansion of human MSC in a microcarrier-based system using commercially available serum-free and xeno-free reagents (StemPro� MSC SFM XenoFree, Life Technologies). After 14 days, BM MSC reached a maximum cell density of 2.0x105 cells/ml (fold increase of 18), while ADSC expanded to 1.4x105 cells/ml (fold increase of 14). Then, the scale-up of this system was successfully achieved for BM MSC in a 1 L fully-controlled stirred bioreactor, reaching a cell density of 1.3�0.1x105 cells/ml  (12-fold increase) after 7 days. The cells maintained tri-lineage differentiation potential and retained the MSC immunophenotypic profile.This system can produce large numbers of high quality MSC, representing a feasible and more efficient alternative to the traditional cell expansion protocol for clinical-scale manufacture of MSC.

Scalable expansion of human mesenchymal stem cells using a microcarrier-based system under serum-free and xeno-free conditions

243rd National Meeting (2012)

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