Successful development of extremely sensitive and accurate immunosensor arrays for clinical measurements of biomarker proteins for early detection and monitoring of cancer though poses a formidable challenge promise to lead to reliable on-the-spot cancer diagnosis, improved therapeutic outcomes at lower costs, decreased patient stress, and will facilitate new targeted therapies. Herein we report an ultrasensitive immunosensor based on a glutathione-protected gold nanoparticle (GSH-AuNP) sensor surface. GSH-AuNP were bioconjugated to the primary antibodies (Ab1) and used to capture human IL-8 in a sandwich electrochemical immunoassay coupled to horseradish peroxidase enzyme labels. When combined with novel massively labeled paramagnetic particles for the electrochemical detection of cancer biomarker interleukin 8 (IL-8), we obtained an unprecedented detection limit (DL) of 1 fg mL-1 (100 aM) for IL-8, the lowest protein level yet detected in serum. The immuonsensor was also used to accurately measure IL-8 in HNSCC cell lines with excellent correlation to the standard ELISA method. These GSH-AuNP based immuonsensors show great promise for the fabrication of ultrasensitive biosensor microarrays for point-of-care cancer diagnosis.

Nanostructured immunosensor for attomolar detection of cancer biomarkers using massively labeled superparamagnetic particles

243rd National Meeting (2012)

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