In this work, we examined the interactions of various nanoconstructs containing beta-tricalcium phosphate nanocrystals, hydroxyapatite as well as alpha-tricalcium phosphate nanocrystals with osteosarcoma cells. In order to mimic the extra-cellular matrix, those materials were functionalized with collagen and peptide sequences such as GRGDSP or BMP. The morphologies of the formed nanocomposites were examined by various electron microscopic methods. In some cases, the materials were bound to self-assembled peptide nanotubes. We also attached ellagic acid to the materials to explore its anti-carcinogenic potency on osteosarcoma cells. In addition to conducting cytotoxicity analysis, we also examined the immunological response of the materials in the presence of macrophages. The cytotoxicity analysis revealed that the ellagic acid functionalized conjugates were cytotoxic toward osteosarcoma and its effect was concentration dependent. We further examined the effect of the nanomaterials on the expression of EGFR in osteosarcoma and their implication in pathogenesis.

Interactions of osteosarcoma cells with engineered nanocomposite scaffolds

243rd National Meeting (2012)

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