Factorial design of  experiments (DoE) allows for the simultaneous testing of multiple  variables during screening and optimization of processes and products.   The effectiveness of different screening and optimization experimental  designs for determining optimum formulations for hen egg white lysozyme (HEWL) activity were compared.   The excipients tested were: pH, NaCl, sucrose, polysorbate 80, and  methionine. HEWL enzymatic activity was analyzed via UV spectroscopy of  Micrococcus lysodeikticus cell lysis in plate reader format. The screening experimental designs tested: 2-level full  factorial, fractional factorial, and Plackett-Burman designs, gave poor  fits to the data, most likely due to curvature in the responses. Central  Composite and Box-Behnken response surface designs of the five factors were then compared. Both experimental  designs identified significant curvature in pH and NaCl responses.   There were some differences in significant factors and interactions  identified by the two designs.  Finally, optimized formulations from the two designs will be compared.

Comparison of screening and response surface experimental designs in selecting an optimized formulation for HEWL activity

243rd National Meeting (2012)

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