This article puts nano-technology, biological chip technology and magnetic particles array technology together, and develops the molecular diagnosis method which fits individual medical treatment demand. Meanwhile, it combines the unique fully enclosed detector design with signal scanners, constructing automated molecular diagnostic system for personal medicine based on multiplex PCR with fully enclosed, automation and high practical. This system takes 96-well PCR plate as the reaction carrier, including mechanical arm, heating and cooling platform, fluorescence detection platform etc. It can realize the whole process automatically from genomic DNA extraction, target sequence amplification, hybridization to fluorescent signal detection. It breaks through the technical bottleneck of the traditional multiplex PCR technique technologies, with high sensitivity, specificity and high yield characteristics, and it is applicable to the high throughput analysis and diagnosis.

Automated molecular diagnostic system for personal medicine based on magnetic separation

243rd National Meeting (2012)

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