DiART is an isobaric, peptide mass-tagging reagent which facilitates relative protein quantitation in a functionally similar manner to well established iTRAQ and TMT reagents. Unlike iTRAQ and TMT reagents, DiART reagents harbor deuterium isotopes. Incorporating deuterium, instead of carbon and nitrogen isotopes, significantly reduces the number of synthesis steps and hence reduces the cost of these reagents. By carefully controlling the position and the number of deuterium labels, DiART reagents have been shown to be devoid of deleterious isotope effects like chromatographic shifts associated with deuterium labels during reverse phase chromatography. Here, we test the applicability of DiART reagents using model protein mixtures and complex fungal cell-wall protein extract using a pulsed Q dissociation (PQD) fragmentation method on a linear ion trap mass spectrometer. We conclude that DiART reagents can be used to accurately and reproducibly perform quantitative proteomic analysis using PQD mode of fragmentation at a significantly lower cost.

Applicability of novel and cost effective 6-plex  isobaric tagging reagent, DiART using model protein mixtures on a linear ion trap (LTQ Xl) mass spectrometer

243rd National Meeting (2012)

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