Bacterial cellulose (BC), produced by Acetobacter Xylinum, consists of ribbon-shaped nanofibers in network-shaped structures. Because of its potential beneficial biological activities, it has been proposed as a biomaterial having a range of biomedical and industrial applications. Human adipose-derived stem cells (hASCs) can differentiate into various mesodermal cell types, including osteoblasts, myoblasts, chondroblasts, and preadipocytes. This study is to explore the feasibility of using hASCs and BC as seeding cells and scaffold in the field of bone tissue engineering. Human liposuction aspirates were proteolytically digested and centrifuged to obtain hASCs. After primary culture in control media and expansion to three passages, the cells were seeded in BC and cultured in osteogenic media. Osteogenesis was assessed by von Kossa, alizarine red and ALP cellular staining following osteogenic induction. The results shows that BC has a unique nano-scale ultrastructure, hASCs proliferate and adhere well on its surface, which is suggestive of a good biocompatibility; hASCs could undergo a successful osteogenic differentiation on BC membrane. In conclusion, the authors identified an osteogenic potential of hASCs a nd demonstrated osteogenic differentiation of hASCs into an osteogenic lineage in three-dimensional BC scaffolds.

Study of osteogenesis differentiation of hASCs in bacterial cellulose

243rd National Meeting (2012)

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