Artificial graft materials are important for bone tissue engineering. Novel tricomponent scaffolds of Chitosan/natural Hydroxyapatite with Chondroitin Sulfate (Chitosan/HAp-CS) and Amylopectin (Chitosan/HAp-AP) have been developed for the first time via freeze-drying method and physicochemically characterized as bone graft substitutes. Chemical interactions and dispersion of HAp, CS and AP in Chitosan matrix have been evaluated. The porosity and water uptake/retention ability of the composite scaffolds decreased whereas thermal stability increased as compared to Chitosan scaffold. The pore size of the Chitosan/HAp, Chitosan/HAp-CS and Chitosan/HAp-AP scaffolds varied from 60-180 �m, 60-400 �m and 80-500 �m, respectively. Cell proliferation, alkaline phosphatase activity and production of Type-1 collagen checked in vitro using MG-63 cell line was higher in composite scaffolds . Excellent thermal stability, interconnected porosity, controlled biodegradation and enhanced cell proliferation observations suggest that the novel Chitosan/HAp-CS and Chitosan/HAp-AP scaffolds are promising biomaterials for bone tissue engineering.

Preparation and characterization of chitosan/natural hydroxyapatite with chondroitin sulfate and amylopectin scaffold for bone tissue engineering

243rd National Meeting (2012)

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