Glycan arrays have become powerful high-throughput tool for examining binding interactions of carbohydrates with lectins, antibodies, cells, and viruses. Recently, it has been applied for antibody detection and profiling, vaccine development, biomarker discovery, and drug screening applications. We report here a new type of glycan array, an oriented and density controlled glycopolymer microarray, fabricated based on end-point immobilization of glycopolymer that was imprinted with boronic acid ligands in different sizes. Briefly, O-cyanate chain-end functionalized lactose-containing glycopolymer was pre-modified by lysozyme-boronic acid, BSA-boronic acid and polyacrylamid-boronic acid ligands and then immobilized onto an amine-functionalized glass slide via isourea bond formation, followed by releasing the boronic acid ligands, respectively. The imprinted glycol-marcroligands showed enhanced lectin (Arachis hypogaea) binding compared to non-imprinted one. Furthermore, SPR results confirmed the same trend of density-dependent lectin binding as glycoarray. This glycoarray provides multidimensional glycan arrays with enhanced performance for probing the ligand specificities of glycan-binding molecules.

Glyco-macroligand microarrays with controlled orientation and varying carbohydrate density exhibit enhanced sensitivity and selectivity

243rd National Meeting (2012)

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