Cell adhesion onto biomaterial surfaces are mediated by adsorbed protein layer. Cell adhesive proteins such as fibronectin (Fn) and vitronectin (Vn) plays an important role in cell adhesion. To elucidate the effect of surface chemistry on adsorption of cell adhesive proteins and subsequent cell adhesion, we examined adsorption of Fn and Vn and cell adhesion to self-assembled monolayers (SAMs) of alkanethiols carrying methyl (CH3), hydroxyl (OH), carboxylic acid (COOH) and amine (NH2). Larger amount of Fn and Vn was adsorbed to COOH- and NH2-SAMs than to CH3- and OH-SAMs in both mixture with bovine serum albumin (BSA) and 2% fetal bovine serum, indicating that Fn and Vn is preferentially adsorbed to these SAMs. Human umbilical vein endothelial cells adhered to COOH- and NH2-SAMs preadsorbed with Fn or Vn even in the presence of 1000-times BSA. These results suggest that competitive adsorption of cell adhesive proteins greatly affects subsequent cell adhesion.

Effect of surface functional group on adsorption of cell adhesive proteins and subsequent cell adhesion using self-assembled monolayers

243rd National Meeting (2012)

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