The ability to engineer DNA-mediated assembly of gold nanoparticles and enzyme-mediated cutting of the assembly is important for developing effective methods in cancer detection. In this presentation, recent findings of our investigations of the assembly of gold nanoparticles by DNA complimentary strand binding and cutting of the double strand DNAs in the nanoparticle assembly by restriction enzyme will be discussed.  The size effect of the nanoparticles on the assembly and cutting was probed.  Surface enhanced Raman scattering (SERS) technique was used to detect the inter-particle �hot spots� in association with the assembly of nanoparticles due to DNA complimentary strand binding.  In addition to traditional assays of the cutting of the assembly by restriction enzymes, both SERS and agarose gel electrophoresis to monitor the cutting process.  New insights into the control of the assembly and cutting processes will be discussed.

Tuning DNA-assembly of gold nanoparticles and enzyme-cutting of the assembly

244th National Meeting (2012)

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