We have recently developed and implemented an Encoded Nanocarrier based on DNA-capped gold nanoparticles (NPs).  Using the known DNA-intercalating properties of many chemotherapy agents, we have designed NPs to load up to 200 drugs/NP, using sequence specific intercalation.  For example, the frontline chemotherapy drugs doxorubicin (DOX) and actinomycin D (ActD) have been employed.  The mechanism for cooperative binding, and the relative binding constants have been probed by monitoring the melting transition of the dsDNA capping, by the swelling of the hydrodynamic diameter by DLS, and via gel electrophoresis.  The cytotoxicity of these encoded nanocarriers was also evaluated using neuroblastoma cancer cells.  These results, and recent work on a multifunctional second-generation system will also be discussed.

DNA-capped nanoparticles as encoded nanocarriers for intercalating chemotherapy drugs

244th National Meeting (2012)

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