The combination of targeted molecular imaging with two-photon fluorescence microscopy (2PFM) provides a powerful technique for chemical biology and, potentially, for non-invasive diagnosis and monitoring of treatment efficacy of a number of diseases, including cancer. The synthesis, photophysical studies, and bioimaging are reported for a versatile norbornene-based block copolymer multifunctional scaffold with a poly(ethylene glycol) block to impart hydrophilicity and biocompatibility, and a block containing two-photon fluorescent dyes and targeting peptide moieties. Bioconjugate polymers, containing a fluorescent dye and a cRGDfK peptide, formed a spherical micelle structure in water of about 90 nm diameter and was analyzed by TEM microscopy. Cell-viability and 2PFM imaging of the new probes with human epithelial U87MG cell lines that over express av�3 integrin and an animal model, along with negative control studies using MCF-7 breast cancer cells and blocking experiments, are reported and indicated high biocompatibility and selectivity of the probes.

Norbornene-based polymer probes for two-photon fluorescence bioimaging

244th National Meeting (2012)

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