Dendritic cells (DCs) are professional antigen-presenting cells that serve as messengers between innate and adaptive immunity.  Prior to acute infection and inflammation, a relatively low number of DCs circulate in peripheral tissues.  Capture of antigens by DCs results in DC maturation and migration to secondary lymphoid organs, where they present processed antigens to na�ve T cells.  This interaction is partially facilitated by the DC surface receptor, DC-specific intracellular adhesion molecule-3 grabbing non-integrin (DC-SIGN).  DC-SIGN recognizes antigens bearing mannose-rich or fucosylated glycans, such as those found in Lewis blood group antigens, including Lewis X (LeX). Here, we report the fabrication of multivalent LeX-functionalized magnetic nanoparticles using the Cu (I)-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. The resulting nanoparticles are selective and biocompatible, serving as a highly efficient tool for DC detection and enrichment.

Detection and isolation of dendritic cells using Lewis-X-functionalized magnetic nanoparticles

244th National Meeting (2012)

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