Heparin is and anticoagulant drug and the related heparan sulfate (HS) is involved in cell differentiation, signaling, inflammatory response, blood coagulation and pathogen invasion. We are  synthesizing both nonasaccharide and decasaccharide libraries on 4-nitrophenyl-�-D-glucuronic acid (PNPG) and GlcUA-AnMan-N3 acceptors using glycosyl transferases and uridine diphosphate (UDP)-N-acetylglucosamine, UDP-N-trifluoroacetylglucosamine and UDP-glucuronic acid donors. HS biosynthetic enzymes, including O-sulfotransferases and N-sulfotransferase, and C5-epimerase, are then used to partially or completely (see structure) modify these oligosaccharides to afford heparin/HS libraries.

Chemoenzymatic synthesis of heparin oligosaccharide libraries

244th National Meeting (2012)

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