Coupling self assembled monolayer (SAM) techniques with biodiagnostic applications has led to custom made electrochemical sensors that can be produced with relative ease, in multiplexed formats, and at low cost. For example, DNA monolayers have been used to detect complementary sequences within complex sample matrices as well as to elucidate the thermodynamic and kinetic parameters of binding various species (e.g. proteins, small molecules) to DNA. Here, electroactively labeled, double stranded DNA (dsDNA) monolayers are interrogated using alternating current voltammetery (ACV) to monitor association between a transcription factor protein and the monolayer . Various ACV input frequencies are tested to determine those most sensitive to protein binding, and concentration series are performed to generate Langmuir-type binding isotherms for quantitative determination of binding affinities. Because the DNA binding reaction is coupled to protein dimerization in solution a model that accounts for coupling between the two equlibria was needed to fully characterize the experimental data. This presentation will discuss optimization of the experimental approach as well as model-based extraction of thermodynamic parameters for protein-DNA interactions using a classical biomolecular systems derived from bacteriophage lambda. .

Modeling protein-DNA interactions at electrified interfaces

244th National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.