Biomaterial associated infections are an increasingly occurring problem in modern healthcare. Combined with the rise of multiresistant bacterial strains they form a serious threat. Development of an antimicrobial coating that remains active for a prolonged time in vivo is therefore desired.Here we report on the synthesis of short, stabilized antimicrobial peptides and their immobilization into hydrogels.We found that several stabilized mimics of the recently described HHC-10 (H-KRWWKWIRW-NH2) show low �M activity against a range of bacteria, making them suitable for immobilization via thiol-ene chemistry. Adding this peptide to a mixture of acrylate-PEG and a thiol-crosslinker afforded a hydrogel coating on PET surface. Subsequent testing of the antimicrobial activity in a JIS-test showed the complete killing of the inoculum, also after washing.This proof of concept will allow us to attach a wide range of active peptides and test them in vivo to prevent biomaterial associated infections.

Stabilized antimicrobial peptide coatings

244th National Meeting (2012)

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