Glutathione disulfide (GSSG) is the oxidized form of glutathione (GSH). The thiol redox state reflecting oxidative stress in the biological system is often represented by the ratio of GSH/GSSG. Thus GSSG is a marker for oxidative stress.  Although oxidative stress has been associated with various diseases, the significance of elevated levels of GSSG remains poorly understood due to a lack of methods to selectively increase intracellular GSSG. Current methods to increase intracellular GSSG include delivering GSSG using its methyl ester form or through inhibition of glutathione reductase. Drawbacks have been noted for both methods. Here we would like to report development of positively charged liposomes that can deliver GSSG into cells resulting in a ~25-time increase in the intracellular GSSG concentration. The details of the preparation and characterization of liposomes, and the delivery into the cells will be presented.

Positively charged liposomes for delivery of glutathione disulfide

244th National Meeting (2012)

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