As a tool for a drug , the preparation of liposomes has been examined for several decades. We investigated to encapsulate lysosomal enzymes extracted from Saccharomyces cerevisiae in liposomes, which are made with L-a-phosphoatidylcholine from egg yolk. We mixed lipid, cholesterol and lysosomal membrane protein isolated from lysosome treated by H2O2 and NH4Cl to fuse liposome surface with various ratio for antimicrobial activity before and after making liposome. The residue after evaporation was mixed using lysosomal enzymes with same ratio and was vortexed to encapsulate them for 1 min. Large lamellar particles were sonicated to make a small unilamellar vesicles for 5 sec. The liposomes were confirmed as cell counts against Escherichia coli to evaluate the antimicrobial activity of lysosomal enzymes. The results showed that the antimicrobial activity was not shown without fusion but fused liposome had antimicrobial activity. Therefore, lysosomal membrane for targeting bacteria will play an important a role.

Preparation of fused liposomes encapsulated lysosomal enzymes for antimicrobial activity

244th National Meeting (2012)

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