Using a newly synthesized gibberellin analog (GA3-AM) and its binding proteins, we developed a novel and efficient chemically induced dimerization (CID) system, that is completely orthogonal to the existing rapamycin-mediated protein dimerization . Combining the two systems should allow applications that were difficult or impossible with only one CID system. By using both chemical inputs (rapamycin and GA3-AM), we designed and synthesized Boolean logic gates in living mammalian cells. These gates produced output signals such as fluorescence and membrane ruffling on a timescale of seconds, a significant improvement over previous intracellular logic gates. The use of two orthogonal dimerization systems in the same cell also allows for finer modulation of protein perturbations than is possible with a single dimerizer.

Rapid and orthogonal logic gating with a novel gibberellin-induced dimerization system

244th National Meeting (2012)

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