Lanthanide luminescent probes are attractive alternatives to radioisotopic and fluorescent labeling due to extremely high detection sensitivity. The probes include antenna fluorophore that absorbs the excitation light and transfers the energy to a lanthanide ion coupled to an antenna through a chelating group as well as crosslinking group for the attachment to a biomolecule of interest. The excited lanthanide state is long-lived that allows to avoid short-lived background fluorescence using time-gated detection. We developed new efficient strategies for the synthesis of lanthanide probes, which makes them available for variety of biological, biomedical and technical applications. The synthesized probes were 50-100 times more sensitive comparing to conventional fluorescent labels in the context of molecular beacon hybridization probes and allowed highly contrast microscopic imaging of bacterial and mammalian cells. The probes with Eu3+, Tb3+, Dy3+, and Sm3+ lanthanide ions were characterized using fluorescent, NMR, light absorption as well as laser pulsed spectroscopy.

Development of long lived luminescent probes for ultrasensitive detection of biopolymers

244th National Meeting (2012)

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