Characterization of protein complexes by mass spectrometry has been improved by the use of ion mobility (IM).  Unfortunately, collision-induced dissociation often leads to release of only monomer fragments, providing limited information on substructure of a complex.  In the work to be presented here, surface-induced dissociation (SID) is combined with ion mobility in SID-IM and IM-SID combination.  A variety of homo-oligomeric and hetero-oligomeric complexes have been investigated.  In addition, the influence of different solution additives has been explored to determine conditions that provide more native-like structures.  SID is shown to provide fragmentation spectra in which released proteins are still folded, better reflecting the substructure of the complex.

Ion mobility and surface-induced dissociation: Characterization of protein complexes

244th National Meeting (2012)

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