Phage display technology is an effective technique for the isolation of the peptides and proteins, that can bind to a specific molecule. This approach allows the use of chemicals in physiological conditions as the target, so phage display is suitable for the development of peptides that bind to cell surface antigen, because native proteins can be used as the target in physiological conditions. In this research, the undifferentiated P19 embryonal carcinoma cells (P19 cells) were used as the model of stem cells, and the isolated peptides could bind to the undifferentiated P19 cells from phage display library. The isolated phage selectively binds to the undifferentiated P19 cells, and the phage binding was inhibited by the free peptides. Furthermore, the drug screening to find the nerve growth factor using high-throughput micro-chamber array chip and phage display technology was attempted.

Application of the phage display technology to screening and detecting of an undifferentiated embryonal carcinoma stem cell and a nerve growth factor

244th National Meeting (2012)

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