A surface plasmon resonance (SPR) biosensor method was developed for detection of staphylococcal enterotoxins A (SEA) and B (SEB) in cultures after removal of Protein A with affinity chromatography.  Protein A is also produced in bacterial cultures interferes with immunoassay techniques resulting in inaccurate detection of SEA or SEB and fouling of the sensors.  Four strains of Staphylocccocus aureus were cultured and the toxins were analyzed with a SPR biosensor sandwich immunoassay technique.  Analysis of two batches of cultures showed that strain S-6 produced 5.76 �g/mL SEB but also contained 96.5 ng/mL SEA; FRI-100 produced 2.40 �g/mL SEA and 27.88 ng/mL SEB; FRI-196 produced 801 ng/mL SEA with 88.5 ng/mL SEB; and FDA-196e produced 113.6                 ng/mLSEA with 9.95 ng/mL SEB.  SEA and SEB were co-produced by these strains, and immuno-affinity clean-up allowed at least 5 - 12 separate analysis with RSDs below 12%.  Artificially inoculated food samples will be analyzed using these procedures.

Biosensor detection of staphylococcal enterotoxin A and B in cultures after removal of Protein A with immuno-affinity chromatography

244th National Meeting (2012)

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