By modulating the expression of drug metabolizing enzymes, structurally diverse bioactive food components such as isothiocyanates, selenium, and dithiolthiones, may influence the efficacy of anticancer DNA alkylating agents. The broad goal of this work is to understand how exposures to bioactive food components can alter cellular metabolic capacity and drug cytotoxicity. In this study, the responses of human colon cells to sodium selenite, sulforaphane or dithiolethione were evaluated. Activities and expression levels of targeted reductases were measured by a combination of spectroscopic assays and proteomics measurements. Induced changes in molecular profiles were correlated with drug toxicity outcomes. The results of this study illustrate responses to varied food components, how these compounds may impact drug efficacy, and apply a new experimental tool with potential applications for identifying food components that may influence enzyme expression and/or drug action.

Impact of bioactive food components on drug metabolizing enzymes in human colon cells

244th National Meeting (2012)

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