Metabolites of blood plasma from high-fat diet (HFD) induced obese mice were analyzed using gas chromatography coupled with time-of-flight mass spectrometry (GC-TOF/MS) after fed four different diets, including corn starch (CS), garcinia cambogia (GC), white rice (WR), and black rice (BR) for 8 weeks. GC-TOF/MS data sets were then applied to principal component analysis (PCA), a multivariate analysis, to access metabolic differences between biological specimens. In total, twenty five metabolites, including 4 carbohydrates, 6 lipids, 5 organic acids, and 10 amino acids, were identified in blood plasma of mice fed HFD. The order in the levels of glucose, galactose, and cholesterol among 4 treatment groups was as follows; CS > WR > GC (= BR). The levels of some fatty acids such as propanoic acid, butanoic acid, and hexadecanoic acid significantly increased in plasma of mice in CS group than that obtained from mice in BR group, whereas the level of 9,12-octadecadienoic acid was higher in mice of BR group than CS group (p<0.05). For amino acids, the levels of leucine, proline, and threonine markedly increased in plasma from mice fed CS compare to ones in BR group (p<0.05). A PCA score plot illustrated the separation of samples according to duration of feeding by principal component 1 (PC 1) (29.9 %) and also diet groups by PC 2 (15.8 %). HFD induced obese mice fed four different diets were divided into two groups: BR group vs. CS, GC, and WR groups. The major metabolites contributing to this clustering were mannose, isoleucine, threonine, and phenylalanine.

Metabolic profiling of blood plasma in high-fat diet induced obese mice fed different rice cultivars

244th National Meeting (2012)

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