Theaflavins as an active antioxidant and a natural pigment and pharmacologically active molecule which obtained from black tea were bioprocessed in a packed bed reactor using immobiled laccase(EC1.10.3.2,From Trametes spp).In this study, we used D152(macroreticular weak acidic acrylic acid cation exchange resin) as a matrix to immobolize laccase extracted from Trametes spp.then we utilized this bio-materials to transformat tea catechins into theaflavins consecutively in a packed bed reactor.Four mainly facts were considerated in this experiment as following:temperature,substrate flow ratio,oxygen flow rate,pH and subsrate concentration.As a results,we selected the temperature at 28?,substrate flow ratio 2.0mL/min, oxygen flow rate 55mL/min,pH5.7 and substrate concentration 10mg/mL as a optimized condition to biosynthesize theaflavins at a highly efficiency.This experiment conditions will give a reference for large scale producing theaflavins basically.

Study on continuous biosynthesis of theaflavins by immobilized laccase in a packed bed reactor

244th National Meeting (2012)

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