Oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC) are three approved tetracyclines commonly used to treat bacterical infections in cattle. Tolerance of 12ppm has been established for the sum of the three tetracycline residues in bovine kidney. The current regulatory method for these antibiotics is a microbiological assay which is non-specific, time consuming, and does not provide information on the amount of each drug present when multiple drugs are used to treat an animal. The objective of this study is to validate a faster and specific LC-MS/MS method for the determination and confirmation of the three tetracyclines in bovine kidney and then bridge it to the microbiological method. The new method involves performing liquid and solid phase extraction. Kideney samples are chromatographically resolved with a Polar-RP Synergi column and analyzed by triple quadrupole tandem mass spectrometry. The method has been thoroughly validated according to relevant FDA guidelines.

Determination and confirmation of oxytetracycline, chlortetracycline, and tetracycline in bovine kidney by LC-MS/MS

244th National Meeting (2012)

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