In this presentation we report the results of a column lifetime study of silica-based TSKgel G3000SWxl 5�m, SEC columns with dimension of 7.8mm ID x 30cm; a column-type that is widely used for the separation of monoclonal antibodies. We evaluated the performance of three columns each from three different commercial lots using a phosphate buffer pH 6.7 as mobile phase  and standard protein test-mixture. Although the use of a guard column to protect the analytical column is highly recommended and ought to be part of a standard operating procedure, in practice not all users do so. Thus, to provide extra stress on the column, we chose not to use guard columns. The study shows excellent reproducibility in retention time with a very low percent relative standard deviation (%RSD) of <1% (n=10) within the same lot and between the lots. The column maintained excellent stability and packing integrity over 1000 injections.

Silica-based diol-bonded size-exclusion chromatography column with high lifetime, for protein and antibody separations

244th National Meeting (2012)

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