To reverse the negative effects of Pb2+, an important first step is accurate, on-site and real-time detection and quantification of Pb2+ in the environment or in vivo. Herein, we report a novel biosensor for rapid, highly specific and sensitive detection of Pb2+ based on GR-5 DNAzyme.  In the presence of Pb2+, GR-5 DNAzyme cleaves its specific substrate DNA molecule, which further hybridizes with the complementary strands immobilized on the optic fiber sensor surface. Subsequent fluorescent signals of the hybridized fluorescent labeled fragment provides quantitative information on the concentrations of Pb2+ with a dynamic range from 2 to 40nM with a detection limit of 2.51nM(0.52 ngmL-1). The proposed sensor also shows good selectivity against other mono and divalent metal ions. The sensor was regenerated with 0.5% SDS solution (pH1.9) over 100 times without significant deterioration of the sensor performance. This portable sensor system can be potentially applied for on-site real-time inexpensive and easy-to-use monitoring of Pb2+ in environmental samples such as effluents or water bodies.

DNAzyme based lead (II) ions biosensor development on a portable optic fiber sensing platform

244th National Meeting (2012)

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