We report on the synthesis, functionalization and testing of approximately 25 nm iron oxide nanoparticles that have been optimized for biodetection by magnetorelaxometry. The nanoparticles are synthesized through a method where iron oleate is generated in situ, and is then thermally decomposed. The nanoparticles are functionalized with biocompatible polymers, including poly(N-vinylpyrrolidone) and poly(ethylene glycol), that provide excellent colloidal stability, followed by antibody conjugation. Colloidal stability of individual particles, without the slightest agglomeration is critical in this application and is crucial to the success of the methodology. The particles are thoroughly characterized by TEM, SAXS, AC and DC magnetometry, dynamic light scattering, and FTIR. Finally the performance of the particles in detection of breast cancer via magnetorelaxometry is tested both in vitro and in vivo. While this work is focused on one application, the design is flexible and the general approach is applicable to a variety of magnetic detection approaches.

Design, synthesis, and functionalization of large iron oxide nanoparticles optimized for magnetorelaxometry detection

244th National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.