Abstract: Recently, we successfully developed an efficient synthetic strategy toprepare different 1,2,3-triazolyl dye-conjugate phosphoramidites in largequantities. Using these newly synthesized, conjugated phosphoramidites asbuilding blocks, the new generation of combinatorial fluorescence energy transfer(CFET) tags can be readily constructed on a DNA synthesizer following standardprotocols. Thus far, we have successfully constructed a series of new generationof CFET tags that exhibit enhanced FRET signals, and our synthetic approachexhibits high versatility. The newly synthesized fluorophore-labeled monomerscan be attached to any position on a rigid molecular scaffold in order to yielddistinguishable fluorescent signatures. The new generation of CFET tags can beexcited by a single wavelength of 488 nm and analyzed by a simple opticalsystem. The design, synthesis, purification of CFET tags, and the methods to usethe CFET tags and molecular affinity for sequencing analysis will be discussed.

Novel molecular tags with enhanced fluorescence resonance energy transfer signals for multiplex biological assays

244th National Meeting (2012)

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