An aquatic microbial isolate was obtained from, the Esopus Creek, in the Hudson Valley, NY.  This isolate was biochemically characterized, and identified to be similar to the purple pigment (violacein, 1  ) producing Janthinobacterium.  It has been previously demonstrated that violacein is produced when cells are at a high density, a result of quorum sensing.   Published reports have also identified violacein as a useful antimicrobial agent used in bioremediation.   The genome of this isolate has been sequenced and is currently being assembled and annotated.  Through isolation and characterization of 1  and its derivatives, we are working to elucidate the biochemical pathway of violacein production.  Additionally, the synthesis of 1  and compounds related to 1  are used as an analytical comparison for the characterization and identification of violacein's antimicrobial activity.  These results will provide impetus for the comparison of  violacein's production pathway in additional natural isolates.

Analysis, characterization, and synthesis of violacein from <i>Janthinobacterium</i> isolate extracts

244th National Meeting (2012)

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Analysis, characterization, and synthesis of violacein from Janthinobacterium isolate extracts