In photosynthetic reaction centers (RCs) under illumination, photochemically induced dynamic nuclear polarization (photo-CIDNP) can be observed by 13C and 15N MAS NMR as dramatic increase of signal intensity [1, for review 2]. The solid-state photo-CIDNP effect leads to signal enhancement of more than 80,000 allowing to study RCs directly in membranes, cells and entire plants. The origin of the effect is now understood. Photo-CIDNP-MAS NMR is applied as analytical tool to study the photochemical machinery of various RCs. The effect has also been observed in a blue-light photoreceptor [3], at liquid state [4] and is expected to be more common at low fields [5].[1] Zysmilich & McDermott (1994) JACS 116, 8362.[2] Daviso et al. (2008) In: Biophysical Techniques in Photosynthesis II (Aartsma & Matysik) Springer, pp. 385.[5] Thamarath et al. (2010) JACS 132, 15542.[4] Daviso et al. (2011) JACS 133, 16754.[5] Jeschke et al. (2011) JPCA 115, 9919.

Solid-state photo-CIDNP effect and its analytical application

244th National Meeting (2012)

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