Glycan expression behavior of mammalian cell surfaces provides valuable information for biological functions. Especially, cancerous cells have considerably different from structural formation and over-expression of glycans compared with noncancerous cells. Here, we present a microarray-based glycomics approach consisting of cell patterns and tissue specimen with well-defined area and biotin-lectin conjugated streptavidin-quantum dots (SA-QD). Our approach allows high sensitivity, high signal to noise, and providing quantitative information on surface glycans for each cell line examined including human cancer tissues (breast and colon cancer). Additionally, this assay method performed with patterned cells obtained reliable and consistent quantification data. The results clearly show significant changes in glycosylation for each normal/cancerous cell and tissue. Thus, the cell surface glycan assay using biotin-lectin and SA-QD based on patterned cells provided useful assay tools for high throughput cancer diagnostics and cellular physiological studies.

Surface glycans analysis on live cell microarrays and tissues via lectin-nanoparticle conjugate

244th National Meeting (2012)

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