Backscattering interferometry (BSI) is an emerging analytical technique that detects changes in refractive index on the order of 10-7 RI units based on the binding interactions of two species.  BSI has been successfully employed in examining a wide range of interacting systems in aqueous and non-aqueous solvents.1 In the present study, BSI is employed to examine the interaction of a series of known and novel inhibitors of acetylcholinesterase (AChE).  AChE plays an important role in neurological and inflammatory diseases, so a sensitive, rapid and straightforward inhibitor screening method is of the utmost importance.  At the lowest limit, BSI can detect AChE-inhibitor interactions at 100 pM and avoids false positives with acetylthiocholine substrates seen in colorimetric techniques.  Additionally, the experiments can be carried out in free-solution without the need for fluorescent labels.  Recent advances in the BSI system and new system analyses will be discussed. References (1)    Kussrow, A.; Enders, C.S.; Bornhop, D.J, Anal. Chem. 2012 , 84, 779-792.

Detection of acetylcholinesterase-inhibitor interactions using back-scattering interferometry

244th National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.