This project investigated the antiproliferative effects of metal compounds, specifically those containing gold, copper, and barium, on breast cancer cells (MCF-7), skin cancer cells (A375), and normal Human Foreskin Fibroblasts (HFF).  In separate experiments, cells were treated with concentrations of 1-100 �M of Gold(III) chloride trihydrate, (HAuCl4�3H2O), Copper(II) sulfate pentahydrate (CuSO4�5H2O), and Barium nitrate (Ba(NO3)2).  Cytotoxicity was monitored utilizing Sulforhodomine B (SRB) assays.  Results indicated that MCF-7 cells could tolerate 100 �M HAuCl4�3H2O, but cell death was apparent for A375 (75 �M) and HFF (100 �M) cells.  There were no antiproliferative effects of CuSO4�5H2O on A375 or HFF cells.  However, growth inhibition was observed for MCF-7 cells at 25-100 �M CuSO4�5H2O.  Interestingly, all three cell lines tolerated 100 �M Ba(NO3)2.  Further testing at 150-800 �M Ba(NO3)2 showed growth inhibition for both MCF-7 and A375 cells, but cell death was evident for HFF cells at 800 �M Ba(NO3)2.

Antiproliferative effects of gold, copper, and barium compounds on MCF-7, A375, and HFF cells

244th National Meeting (2012)

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