Clove oils were extracted from clove buds using supercritical CO2, steam distillation and hydrodistillation, and the effect of the clove oils and its major components on selected fungi that caused postharvest decay of fruits was studied. Minimum inhibitory concentration of clove oil extracted by steam distillation on Botrytis cinerea, Alternaria spp. Emericella nidulans (Eid.) and Penicillium expansum was 2.5�L/mL, and minimum bactericidal concentration were 5.0, 5.0, 5.0, 2.5�L/mL, respectively. Among the three extraction methods, antimicrobial activities of the oils in decreasing order was steam distillation, supercritical CO2 , and hydrodistillation. Eugenol and Eugenol acetate were the main antifungal ingredients of clove oil, and had strong inhibitory effect on Botrytis cinerea and Penicillium expansum, while �-caryophyllene could not inhibit the growth of the fungi. �-Caryophyllene did not have any synergistic inhibitory effect on fungi with Eugenol or Eugenol acetate.

Antifungal activity of clove oil extracted by steam distillation, hydrodistillation, and supercritical carbon dioxide

244th National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.