Allergic reactions are caused by an immune response to a substance not otherwise pathogenic. The quantity of allergen required to produce anaphylaxis varies greatly between individuals. Managing, identifying, and preventing trace allergen levels are therefore primary concerns for all food manufacturers. The most typical approach to quantifying proteins are ELISA-based methods; however, this methodology has limitations including high variability, a limited sensitivity range, and issues with antibody specificity. This paper compares the accuracy and sensitivity of LC-MS-MS methodology to an ELISA-based method for Gly m Bd 30k , a typical allergen in soybeans.

Comparison of LC/MS/MS and ELISA methodology for a typical allergen

244th National Meeting (2012)

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