The covalent immobilization of a commercial preparation of cellulase on a reversibly soluble-insoluble enteric polymer Eudragit S-100 by carbodiimide coupling was carried out. The characteristics of Covalent Eudragit-cellulase were evaluated using Gel filtration analysis and UV spectra. Gel filtration analysis revealed that the cellulases were covalently bonded to the polymer.Covalent Eudragit-cellulase had immobilization degree about 40% and the relative activity about 80% at Eudragit S-100 concentration 15 g/L. When cellulase is bound to the polymer, the solubility profile becomes similar to Eudragit's . Enzymatic treatment of cotton yarns using cellulases has been investigated. It was found that using the immobilized cellulase in the enzymatic treatment of cotton there was a reduction of weight and tensile strength loss because the hydrolytic attack is only limited to the surfaces of cotton fibers.

Immobilization of cellulases on the reversibly soluble polymer Eudragit S-100 for cotton treatment

244th National Meeting (2012)

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